CellTiter-Glo Luminescent Cell Viability

Cell Viability & IC50, Outsourced.

ATP luminescence cell viability for compound screening and IC50 determination — automated sigmoidal dose-response fitting with results in JSON/CSV for model ingestion.

3–5 days turnaround

IC50 + CI output

96/384-well plate format

Specifications

What you submit. What you get back.

Turnaround

3–5 business days

Includes cell seeding, compound treatment, viability readout, and data analysis. Cell line sourcing adds 1–2 days if needed.

Sample Requirements

500+ cells per well

96-well or 384-well format. Cell line or primary cells. Compound provided in DMSO stock; minimum 10-point dose range recommended for IC50.

Output Format

JSON + CSV

Raw RLU values, % viability normalized to controls, IC50 with 95% CI, hill slope, curve fit parameters, and plate maps.

AI-Readiness

Dose-response data ready for your models

CTG results from Litmus include the full dose-response curve — not just an IC50 number. Every data point, normalized viability, fitted curve parameters, and confidence intervals are structured so your AI pipeline can consume them without preprocessing.

Automated 4-parameter sigmoidal dose-response fitting
IC50, EC50, and hill slope with 95% confidence intervals
Raw RLU values alongside % viability normalized to vehicle and positive controls
Selectivity ratios computed if run across multiple cell lines
Full plate map with compound IDs, concentrations, and well coordinates

                            # CTG result payload
                            assay: "CTG"
                            compound: "CPD-042"
                            cell_line: "HeLa"
                            ic50_uM: 1.84
                            ic50_ci: [1.41, 2.39]
                            hill_slope: 1.12
                            curve: [
                              { conc_uM: 0.1, viability: 97.2 },
                              { conc_uM: 1.0, viability: 54.8 },
                              ...
                            ]
                        

Use Cases

Who orders CTG assays from Litmus

Compound Toxicity & IC50 Screening

Rapidly determine IC50 values for a compound series to guide SAR decisions and prioritize leads before investing in more complex assays.

Selectivity Profiling

Run CTG across a panel of cancer and normal cell lines to establish a therapeutic window and support lead optimization decisions.

Combination & Synergy Studies

Measure viability responses to drug combinations across dose matrices to identify synergistic or antagonistic interactions.

Ready to run your viability assay?

Tell us your cell line, compound, and number of conditions — we'll scope the plate design and turnaround.

Or email us directly. No spam, ever.