Cell-Based & Phenotypic Assays

Cell-Based Assays, Outsourced.

Programmable cell-based protocols with high-content imaging — custom endpoint design, multiplexed readouts, and structured data output built for computational analysis.

Custom turnaround

HCI imaging available

Multiplexed endpoints

Specifications

What you submit. What you get back.

Turnaround

Custom — scoped per project

Turnaround depends on protocol complexity, cell line, and number of conditions. Scoped during initial call. Typically 5–14 business days.

Sample Requirements

Cell line + compound

Customer-supplied or sourced cell lines. Compound in DMSO stock. Protocol design consultation included — no pre-built SOP required.

Output Format

JSON + CSV + images

Quantified per-cell or per-well measurements, automated image analysis outputs, phenotypic feature vectors, and raw image files.

AI-Readiness

Phenotypic data built for ML pipelines

High-content imaging data is notoriously difficult to handle — proprietary formats, massive file sizes, and inconsistent segmentation. Litmus delivers pre-processed, per-cell feature vectors and aggregated well-level statistics in structured formats that plug directly into phenotypic ML models.

Automated cell segmentation and per-cell feature extraction
Per-well summary statistics (mean, median, %positive, intensity distributions)
Multiplexed fluorescence channel data normalized and background-corrected
Phenotypic feature vectors formatted for direct ML model input
Raw TIFF images and segmentation masks available for custom analysis

                            # Cell-based result payload
                            assay: "cell-based"
                            endpoint: "nuclear translocation"
                            wells: [
                              { id: "B02",
                                cell_count: 412,
                                pct_positive: 73.4,
                                mean_intensity: 2847,
                                features: [...] },
                              ...
                            ]
                            images: "s3://..."
                        

Use Cases

Who orders cell-based assays from Litmus

Target Validation & MOA Studies

Confirm that a compound engages the intended cellular target and produces the expected phenotypic response in a relevant cell model.

Phenotypic Drug Screening

Screen compound libraries for desired phenotypic changes — morphology, localization, signaling state — without requiring prior target knowledge.

High-Content Imaging Panels

Measure multiple cellular endpoints simultaneously — viability, nuclear morphology, organelle health, and pathway activation — in a single multiplexed experiment.

Tell us what you need to measure.

Describe your cell model, compound, and the endpoint you care about — we'll design the protocol and scope the run.

Or email us directly. No spam, ever.