Enzyme-Linked Immunosorbent Assay

ELISA Protein Quantification, Outsourced.

Sandwich ELISA for cytokines, antibodies, and target proteins — with automated 4PL curve fitting and results delivered in JSON/CSV for immediate model ingestion.

3–5 days turnaround

JSON/CSV output format

4PL fit curve fitting

Specifications

What you submit. What you get back.

Turnaround

3–5 business days

From sample receipt to data delivery. Rush available on request.

Sample Requirements

50–100 µL per well

Cell culture supernatant, serum/plasma, or tissue lysate. Minimum sample volume confirmed via Intelligent Manifest before shipment.

Output Format

JSON + CSV

Raw OD values, fitted curve parameters, calculated concentrations, LOD/LOQ, and interplate normalization data.

AI-Readiness

Data your models can actually use

ELISA results from Litmus arrive pre-processed and structured — not as a PDF or raw plate reader export. Every result includes the fitted curve, calculated concentrations, and full experimental metadata in a single JSON payload.

4-parameter logistic (4PL) curve fitting applied automatically to every standard curve
LOD and LOQ calculated and flagged per assay run
Interplate normalization when running multi-plate experiments
Raw OD values included alongside processed concentrations
Full metadata: antibody lot, plate map, operator ID, timestamp

                            # ELISA result payload
                            assay: "ELISA"
                            target: "IL-6"
                            samples: [
                              { id: "S1", conc_pg_mL: 142.3,
                                od: 0.847, in_range: true },
                              ...
                            ]
                            curve_params: { a: 0.08, b: 1.21,
                              c: 118.4, d: 2.94 }
                            lod_pg_mL: 3.9
                            r_squared: 0.9991
                        

Use Cases

Who orders ELISA assays from Litmus

Target Engagement & Biomarker Quantification

Measure circulating or secreted target proteins to confirm compound engagement and dose-response relationships in cellular or in vivo models.

Antibody Titer & Immunogenicity

Quantify antibody levels in serum or cell supernatant for biologic drug development, vaccine response studies, or hybridoma screening.

Cytokine & Inflammatory Profiling

Measure IL-6, TNF-α, IFN-γ, and other cytokines to characterize immune response in oncology, inflammation, and immuno-oncology programs.

Ready to run your ELISA?

Tell us your target, sample type, and volume — we'll handle the rest.

Or email us directly. No spam, ever.